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Journal of Nutrition Vol. 100 No. 12 December 1970, pp. 1377-1382
Copyright © 1970 by American Society for Nutrition
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Causes of the Increased Requirement for Vitamin B12 in Rats Subsisting on an Unheated Soybean Flour Diet1

S. Edelstein and K. Guggenheim

Department of Nutrition, Hebrew University-Hadassah Medical School, Jerusalem, Israel

Possible causes of the increased requirement for vitamin B12 by rats fed a diet of unheated soybean flour were investigated. Decreased intestinal synthesis or increased destruction, as well as decreased absorption from the intestine were excluded. However, the vitamin B12 formed by the intestinal flora was less available for absorption when synthesized by rats subsisting on unheated soybean flour. Furthermore, turnover of vitamin B12 in these rats, as estimated by the rate of disappearance of injected 57Co-labeled vitamin B12 from kidneys, liver and spleen, was slightly accelerated.

1 Supported by a grant from the U. S. Department of Agriculture under P.L. 480.

Manuscript received 15 June 1970.




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