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The Demonstration of Oestrus in the Vitamin A-deficient Rat by Supravital Study of the Vaginal Smears¹

Two Figures

Department of Anatomy, Vanderbilt University School of Medicine, Nashville, Tennessee

1. The lavage method of obtaining smears of the cells of the rat's vagina, stained supravitally with neutral red, makes possible the demonstration of oestrous cycles in vitamin A-deficient animals in which abnormal cornification has become too severe to permit their identification by dry stained smears.

2. The excessive keratinization, which more or less masks the underlying cyclic changes, is a primary response of the vaginal epithelium to lack of vitamin A.

3. The increased lengthening of the cyclic change is indirectly due to associated growth retardation and decline.

Manuscript received 4 December 1934.

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				S. W. CLAUSEN THE PHARMACOLOGY AND THERAPEUTICS OF VITAMIN A JAMA, July 9, 1938; 111(2): 144 - 154. [Abstract] [PDF]

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