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Journal of Nutrition Vol. 1 No. 2 November 1928, pp. 139-153
Copyright © 1928 by American Society for Nutrition
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Vitamin Requirements of Nursing Young

III. A Quantitative Biological Method for the Study of Vitamn B1 Requirements of Nursing Young of the Albino Rat,2

Barnett Sure and Dorothy J. Walker

(From the Department of Agricultural Chemistry, University of Arkansas, Fayetteville, Arkansas)

E. H. Stuart

(From the Research Laboratories of Eli Lilly and Company, Indianapolis, Indiana)

1. A quantitative biological method has been perfected for the vitamin B requirements of nursing young which is sensitive to the extent of one milligram per nursling per day.
2. A vitamin B unit, applied to nursing young, has been defined as follows: The vitamin B unit is the daily amount of vitamin B which must become available to a nursing young of the albino rat weighting 30 grams, in order to permit a gain of 10 grams in 7 to 10 days.
3. One gram of a dehydrated baker's yeast, Federal brand, has been found to contain 7 units of vitamin B. Applying our quantitative biological method of assay to vitamin B concentrates we found variations to the extent that one gram contained as little as 14.4 units and as much as 125 units.

1 The term "vitamin B" is used in this and the paper following to signify the combination of the labile-antineuritic and the stable factors associated with the vitamin B complex, both of which are necessary for growth. We are at present engaged in differentiating the requirements of nursing young with respect to these two dietary factors, and our findings along this line will be presented in subsequent communications.

2 Research paper No. 65, Journal Series, University of Arkansas. Aided by a grant from Eli Lilly and Company, Indianapolis, Ind.

Manuscript received 23 July 1928.




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